ZEB1 antibody (AA 1074-1124)

Catalog No. ABIN7454797

The Rabbit Polyclonal anti-ZEB1 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect ZEB1 in samples from Human and Mouse.

Quick Overview for ZEB1 antibody (AA 1074-1124) (ABIN7454797)

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZEB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-ZEB1 Antibody

Binding Specificity: AA 1074-1124

Purpose: Rabbit anti-ZEB1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1074 and 1124

Isotype: IgG

Application Details

Application Notes:

IHC: 1:200 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for ZEB1

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Alternative Name: ZEB1

Background: Background: ZEB1 (zinc-finger E-box-binding homeobox 1), also known as TCF8 (transcription factor 8), is a delta-EF1/ZFH-1 C2H2-type zinc finger that bears seven C2H2-type zinc fingers and one homeobox DNA-binding domain. ZEB1 is a transcriptional repressor of interleukin-2 (IL-2). Mutations in the ZEB1 gene are associated with posterior corneal dystrophy type 3 (PPC3) a rare disorder characterized by metaplasia and overgrowth of corneal endothelial cells. ZEB1 appears to be critical to epithelial to mesenchymal transitions (EMT) during development and cancer metastasis. As a transcription factor involved in EMT, ZEB1 promotes EMT by repressing genes associated with the epithelial phenotype and activates genes associated with the mesenchymal phenotype.

Gene ID: 6935

NCBI Accession: NP_110378

UniProt: P37275

Pathways: Regulation of Muscle Cell Differentiation