BRCC3 antibody (AA 266-316)

Catalog No. ABIN7454833

The Rabbit Polyclonal anti-BRCC3 antibody is suitable to detect BRCC3 in samples from Human and Mouse. It has been validated for WB, IP and IHC (fp).

Quick Overview for BRCC3 antibody (AA 266-316) (ABIN7454833)

Target: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-BRCC3 Antibody

Binding Specificity: AA 266-316

Purpose: Rabbit anti-BRCC36 Antibody, Affinity Purified

Predicted Reactivity: Orangutan

Purification: Affinity Purified

Immunogen: between AA 266 and 316

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for BRCC3

Target: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Alternative Name: BRCC36

Background: Background: BRCA1/BRCA2-containing complex subunit 36 (BRCC36) is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. BRCC36 is a component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites and leads to targeting of the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, BRCC36 specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) [taken from the Universal Protein Resource (UniProt) P46736].

Gene ID: 79184

NCBI Accession: NP_077308

UniProt: P46736

Pathways: Positive Regulation of Response to DNA Damage Stimulus