TFAM antibody (AA 196-246)

Catalog No. ABIN7454907

This Rabbit Polyclonal antibody specifically detects TFAM in WB, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for TFAM antibody (AA 196-246) (ABIN7454907)

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TFAM antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-TFAM Antibody

Binding Specificity: AA 196-246

Purpose: Rabbit anti-TFAM Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 196 and 246

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for TFAM

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Alternative Name: TFAM

Background: Background: Transcription factor A, mitochondrial (TFAM) binds to the mitochondrial light strand promoter and functions in mitochondrial transcriptional regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase and promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. TFMA is required for maintenance of normal levels of mitochondrial DNA and may play a role in organizing and compacting mitochondrial DNA [taken from the Universal Protein Resource (UniProt) Q00059].

Gene ID: 7019

NCBI Accession: NP_003192

UniProt: Q00059

Pathways: Chromatin Binding