PARN antibody (AA 589-639)

Catalog No. ABIN7454930

This anti-PARN antibody is a Rabbit Polyclonal antibody detecting PARN in WB, IP and IHC (fp). Suitable for Human and Mouse.

Quick Overview for PARN antibody (AA 589-639) (ABIN7454930)

Target: PARN (Poly A Specific Ribonuclease (PARN))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARN antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-PARN Antibody

Binding Specificity: AA 589-639

Purpose: Rabbit anti-PARN Antibody, Affinity Purified

Predicted Reactivity: Orangutan

Purification: Affinity Purified

Immunogen: between AA 589 and 639

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for PARN

Target: PARN (Poly A Specific Ribonuclease (PARN))

Alternative Name: PARN

Background: Background: Poly(A)-specific ribonuclease (PARN) is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. PARN prefers poly(A) as the substrate, hence, it efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. PARN is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons [taken from NCBI Entrez Gene (Gene ID: 5073)].

Gene ID: 5073

NCBI Accession: NP_002573

UniProt: O95453