PHGDH antibody (AA 1-50)

Catalog No. ABIN7455052

The Rabbit Polyclonal anti-PHGDH antibody is suitable to detect PHGDH in samples from Human and Mouse. It has been validated for WB, IP and IHC (fp).

Quick Overview for PHGDH antibody (AA 1-50) (ABIN7455052)

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHGDH antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-PHGDH Antibody

Binding Specificity: AA 1-50

Purpose: Rabbit anti-PHGDH Antibody, Affinity Purified

Predicted Reactivity: Bovine,Pig,Orangutan,Chimpanzee

Purification: Affinity Purified

Immunogen: Between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for PHGDH

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Alternative Name: PHGDH

Background: Background: D-3-phosphoglycerate dehydrogenase (PHGDH) is an enzyme involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptom [taken from NCBI Entrez Gene (Gene ID: 26227)].

Gene ID: 26227

NCBI Accession: NP_006614

UniProt: O43175

Pathways: Metabolism of Steroid Hormones and Vitamin D, Warburg Effect