KAP1 antibody (AA 1-50)

Catalog No. ABIN7455078

This Rabbit Polyclonal antibody specifically detects KAP1 in WB, IP, ICC and IHC (fp). It exhibits reactivity toward Human and Mouse.

Quick Overview for KAP1 antibody (AA 1-50) (ABIN7455078)

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-KAP1 Antibody

Binding Specificity: AA 1-50

Purpose: Rabbit anti-KAP-1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

ICC: 1:500 - 1:2,500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name: KAP-1

Background: Background: KAP-1 (KRAB-Associated Protein 1) is a member of the tripartite motif RBCC/Trim domain family that contains a RING finger domain, B boxes, and an alpha helical coiled coil region. KAP-1 functions as a corepressor by interacting with the KRAB domain of KRAB zinc-finger transcriptional repressors. KAP-1 also appears to function as a scaffold for chromatin-remodeling complexes involved in transcriptional repression.

Gene ID: 10155

NCBI Accession: NP_005753

UniProt: Q13263

Pathways: Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus