Recombinant MLH1 antibody (AA 450-500)

Catalog No. ABIN7455102

This Rabbit Monoclonal antibody specifically detects MLH1 in WB, ICC, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Recombinant MLH1 antibody (AA 450-500) (ABIN7455102)

Target: MLH1 (MutL Homolog 1 (MLH1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MLH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: BLR112H

Product Details anti-MLH1 Antibody

Binding Specificity: AA 450-500

Purpose: Rabbit anti-MLH1 Recombinant Monoclonal Antibody [BLR112H]

Immunogen: between AA 450 and 500

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 6 μL/1 mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 01:01,00

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Alternative Name: MLH1

Background: Background: MLH1 is an integral component of the DNA mismatch repair (MMR) system that functions to recognize aberrant structures and signals for the repair of single base mismatches or loops in replicated DNA. MLH1 can heterodimerize with DNA mismatch repair protein MLH3 and with mismatch repair endonuclease PMS2. [taken from NCBI Entrez Gene (Gene ID: 4292)].

Gene ID: 4292

NCBI Accession: NP_000240

UniProt: P40692

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response