Recombinant BMI1 antibody (AA 250-300)

Catalog No. ABIN7455106

The Rabbit Monoclonal anti-BMI1 antibody has been validated for WB, IHC (fp), IP and ICC. It is suitable to detect BMI1 in samples from Human and Mouse.

Quick Overview for Recombinant BMI1 antibody (AA 250-300) (ABIN7455106)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: BLR119H

Product Details anti-BMI1 Antibody

Binding Specificity: AA 250-300

Purpose: Rabbit anti-BMI1 Recombinant Monoclonal Antibody [BLR119H]

Immunogen: between AA 250 and 300

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 6 μL/1 mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 01:01,00

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI1

Background: Background: BMI1 (B lymphoma Mo-MLV insertion region 1 homolog) is a core component of the polycomb group (PcG) multiprotein PRC1 complex which plays an important role in transcriptional repression of gene expression during development. BMI1 association with RING protein 1B is important to the E3 ligase activity of RING1B in the PRC1 complex. BMI1 is an oncogene whose overexpression is associated with many types of human tumors and is a stem-cell marker and regulator of stem cell self-renewal.

Gene ID: 648

NCBI Accession: NP_005171

UniProt: P35226

Pathways: Cell Division Cycle, Autophagy