Recombinant PARP1 antibody (AA 215-265)

Catalog No. ABIN7455116

This anti-PARP1 antibody is a Rabbit Monoclonal antibody detecting PARP1 in WB, IP, ICC and IHC (fp). Suitable for Human.

Quick Overview for Recombinant PARP1 antibody (AA 215-265) (ABIN7455116)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: BLR183J

Product Details anti-PARP1 Antibody

Binding Specificity: AA 215-265

Purpose: Rabbit anti-Cleaved PARP1 Recombinant Monoclonal Antibody [BLR183J]

Immunogen: between AA 215 and 265

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 6 μL/mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 17:40:00

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP1

Background: Background: PARP1 (Poly-ADP-ribose polymerase 1) is a member of PARP family of enzymes that transfer the ADP-D-ribosyl group of NAD(+) to an acceptor carboxyl group on proteins. PARP1 catalyzes the poly-ADP-ribosylation of histone and non-histone proteins in response to DNA damage. The over-activation of PARP-1 in response to DNA damage has been shown to promote cell and tissue injury. This observation has initiated research into the therapeutic potential of PARP1 inhibitors in the treatment of cancer, cardiovascular disease, and brain injury.

Gene ID: 142

NCBI Accession: NP_001609

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location