Recombinant SMAD4 antibody (AA 1-50)

Catalog No. ABIN7455159

This Rabbit Monoclonal antibody specifically detects SMAD4 in WB, IP, ICC, FACS and IHC (fp). It exhibits reactivity toward Human and Mouse.

Quick Overview for Recombinant SMAD4 antibody (AA 1-50) (ABIN7455159)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: BLR133J

Product Details anti-SMAD4 Antibody

Binding Specificity: AA 1-50

Purpose: Rabbit anti-SMAD4 Recombinant Monoclonal Antibody [BLR133J]

Immunogen: between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 6 μL/1 mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 01:01,00

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Phosphate Buffered Saline (PBS) containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4

Background: Background: The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD4 forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. SMAD4 functions in the transforming growth factor-beta signaling pathway as well as the bone morphogenetic protein signaling pathway, regulating cell proliferation, angiogenesis, and increasing blood vessel hyperpermeability. [taken from NCBI Entrez Gene (Gene ID: 4089)].

Gene ID: 4089

NCBI Accession: NP_005350

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy