Recombinant ZEB1 antibody (AA 750-800)

Catalog No. ABIN7455173

This anti-ZEB1 antibody is a Rabbit Monoclonal antibody detecting ZEB1 in IHC, ICC, FACS and IP. Suitable for Human and Mouse.

Quick Overview for Recombinant ZEB1 antibody (AA 750-800) (ABIN7455173)

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ZEB1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: BLR217K

Product Details anti-ZEB1 Antibody

Binding Specificity: AA 750-800

Purpose: Rabbit anti-ZEB1 Recombinant Monoclonal Antibody [BLR217K]

Purification: Purified

Immunogen: between AA 750 and 800

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 to 1:500. Epitope retrieval with citrate Buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 6 μL/1 mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate Buffer pH 6.0 is recommended for FFPE cell sections.

WB: 01:01,00

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8 °C

Expiry Date: 12 months

Target Details for ZEB1

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Alternative Name: ZEB1

Background: ZEB1 (zinc-finger E-box-binding homeobox 1), also known as TCF8 (transcription factor 8), is a delta-EF1/ZFH-1 C2H2-type zinc finger that bears seven C2H2-type zinc fingers and one homeobox DNA-binding domain. ZEB1 is a transcriptional repressor of interleukin-2 (IL-2). Mutations in the ZEB1 gene are associated with posterior corneal dystrophy type 3 (PPC3) a rare disorder characterized by metaplasia and overgrowth of corneal endothelial cells. ZEB1 appears to be critical to epithelial to mesenchymal transitions (EMT) during development and cancer metastasis. As a transcription factor involved in EMT, ZEB1 promotes EMT by repressing genes associated with the epithelial phenotype and activates genes associated with the mesenchymal phenotype.

Gene ID: 6935

UniProt: P37275

Pathways: Regulation of Muscle Cell Differentiation