Recombinant LAG3 antibody (AA 23-450)

Catalog No. ABIN7455196

The Rabbit Monoclonal anti-LAG3 antibody has been validated for WB, IHC, ICC, IP, FACS and IF (p). It is suitable to detect LAG3 in samples from Human.

Quick Overview for Recombinant LAG3 antibody (AA 23-450) (ABIN7455196)

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This LAG3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: BLR028F

Product Details anti-LAG3 Antibody

Binding Specificity: AA 23-450

Purpose: Rabbit anti-LAG3 Recombinant Monoclonal Antibody [BLR028F]

Purification: Purified

Immunogen: residues 23-450 (ECD)

Isotype: IgG

Application Details

Application Notes:

IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 6 μL/0.5 mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 17:40:00

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8 °C

Expiry Date: 12 months

Target Details for LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: LAG3

Background: Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4 [taken from NCBI Entrez Gene (Gene ID: 3902)]

Gene ID: 3902

UniProt: P18627

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints