Recombinant EpCAM antibody (AA 264-314)

Catalog No. ABIN7455204

This Rabbit Monoclonal antibody specifically detects EpCAM in WB, FACS, IP, IHC (fp), ICC and IF (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for Recombinant EpCAM antibody (AA 264-314) (ABIN7455204)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: BLR077G

Product Details anti-EpCAM Antibody

Binding Specificity: AA 264-314

Purpose: Rabbit anti-EpCAM Recombinant Monoclonal Antibody [BLR077G]

Immunogen: between AA 264 and 314 (C-term)

Isotype: IgG

Application Details

Application Notes:

IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 12 μL/1 mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 17:40:00

Restrictions: For Research Use only

Handling

Concentration: 500 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: EpCAM

Background: Background: EpCAM, or epithelial cell adhesion molecule, is a type I transmembrane protein expressed on most normal epithelial cells and gastrointestinal carcinomas. It functions as a homotypic calcium-independent cell adhesion molecule. EpCAM is being used as a target for immunotherapy treatment of some human carcinomas [taken from NCBI Entrez Gene (Gene ID: 4072)].

Gene ID: 4072

NCBI Accession: NP_006197

UniProt: P16422