IDH2 antibody (AA 402-452)

Catalog No. ABIN7455220

The Rabbit Polyclonal anti-IDH2 antibody has been validated for WB, IP, IF/ICC and IHC (fp). It is suitable to detect IDH2 in samples from Human and Mouse.

Quick Overview for IDH2 antibody (AA 402-452) (ABIN7455220)

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-IDH2 Antibody

Binding Specificity: AA 402-452

Purpose: Rabbit anti-IDH2 Antibody, Affinity Purified

Predicted Reactivity: Pig

Purification: Affinity Purified

Immunogen: Between AA 402 and 452

Isotype: IgG

Application Details

Application Notes:

ICC-IF: 1:500 - 1:2,500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for IDH2

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Alternative Name: IDH2

Background: Background: Isocitrate dehydrogenase 2 (NADP+), mitochondrial (IDH2) plays a role in intermediary metabolism and energy production. IDH2 may tightly associate or interact with the pyruvate dehydrogenase complex [taken from the Universal Protein Resource (UniProt) P48735].

Gene ID: 3418

NCBI Accession: NP_002159

UniProt: P48735

Pathways: Warburg Effect