ICAM1 antibody (PE-Cy5)

Catalog No. ABIN7457215

The Rat Monoclonal anti-ICAM1 antibody has been validated for FACS. It is suitable to detect ICAM1 in samples from Mouse.

Quick Overview for ICAM1 antibody (PE-Cy5) (ABIN7457215)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is conjugated to PE-Cy5

Application: Flow Cytometry (FACS)

Clone: YN1-1-7-4

Product Details anti-ICAM1 Antibody (PE-Cy5)

Isotype: IgG2b, kappa

Application Details

Application Notes: Check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10^6 cells in 100 μL volume].

Comment:

Matching Isotype Control: PE/Cyanine5 Rat IgG2b, κ Isotype Control (ABIN7469862)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS with 0.05 % Proclin300, 1 % BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: CD54

Background: Intercellular adhesion molecule 1,Icam1,MALA-2,MyD10,CD54,Icam-1,CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.

Gene ID: 15894

UniProt: P13597

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling