BTLA antibody (PE-Cy7)

Catalog No. ABIN7457492

This anti-BTLA antibody is a Rat Monoclonal antibody detecting BTLA in FACS. Suitable for Mouse.

Quick Overview for BTLA antibody (PE-Cy7) (ABIN7457492)

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This BTLA antibody is conjugated to PE-Cy7

Application: Flow Cytometry (FACS)

Clone: PK18-6

Product Details anti-BTLA Antibody (PE-Cy7)

Isotype: IgG1, kappa

Application Details

Application Notes: Check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10^6 cells in 100 μL volume].

Comment:

Matching Isotype Control: PE/Cyanine7 Rat IgG1, κ Isotype Control (ABIN7469825)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS with 0.05 % Proclin300, 1 % BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.

Target Details for BTLA

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Alternative Name: CD272

Background: B- and T-lymphocyte attenuator,Btla,B- and T-lymphocyte-associated protein,CD272,Btla,CD272, also known as B and T lymphocyte attenuator (BTLA), is an Ig superfamily co-inhitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4. BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand herpes virus entry mediator (HVEM) is critical for negatively regulating immune response. The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, and reduced effectiveness of T regulatory cells. BTLA plays an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo. Tolerant T cells have significantly higher expression of BTLA compared with effectors and naive T cells. BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity. It was reported that BTLA may regulate T cell function by binding to B7-H4, but further studies are needed to confirm. The existence of three distinct BTLA alleles has been reported.

Gene ID: 208154

UniProt: Q7TSA3

Pathways: Cancer Immune Checkpoints