SELPLG antibody

Catalog No. ABIN7457880

This Rat Monoclonal antibody specifically detects SELPLG in FACS and BR. It exhibits reactivity toward Mouse.

Quick Overview for SELPLG antibody (ABIN7457880)

Target: SELPLG (Selectin P Ligand (SELPLG))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This SELPLG antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Reagent (BR)

Clone: 4RA10

Product Details anti-SELPLG Antibody

Purification: Purified

Sterility: 0.2 μm filtered

Endotoxin Level: Low Endotoxin: Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Matching Isotype Control: AF/LE Purified Rat IgG1, κ Isotype Control (ABIN7469818)

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE)

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store at 2~8°C. Do not freeze.

Target Details for SELPLG

Target: SELPLG (Selectin P Ligand (SELPLG))

Alternative Name: CD162

Background: P-selectin glycoprotein ligand 1,Selplg,PSGL-1,Selectin P ligand,CD162,The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

Gene ID: 20345

UniProt: Q62170