HMGB1 antibody (N-Term)

Catalog No. ABIN7464144

This Mouse Monoclonal antibody specifically detects HMGB1 in WB, IF, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for HMGB1 antibody (N-Term) (ABIN7464144)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Clone: GT412

Product Details anti-HMGB1 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse, Rat

Purification: Affinity purified by Protein G.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary.

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , mouse brain , rat brain , HMGB1-transfected 293T

Validation: KO/KD, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.79 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: high mobility group box 1

Background: High mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1,DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Molecular Weight: 25 kDa

Gene ID: 3146

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome