HMGA1 antibody (N-Term)

Catalog No. ABIN7464188

This anti-HMGA1 antibody is a Rabbit Polyclonal antibody detecting HMGA1 in WB, ICC and IF. Suitable for Human.

Quick Overview for HMGA1 antibody (N-Term) (ABIN7464188)

Target: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: KO Validated

Product Details anti-HMGA1 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human HMGA1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.87 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for HMGA1

Target: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Alternative Name: high mobility group AT-hook 1

Background: High mobility group AT-hook 1 , HMG-R , HMGA1A , HMGIY,This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Molecular Weight: 12 kDa

Gene ID: 3159

UniProt: P17096

Pathways: Nuclear Hormone Receptor Binding, Autophagy