ME1 antibody

Catalog No. ABIN7464215

The Mouse Monoclonal anti-ME1 antibody (Clone GT15611) (ABIN7464215) specifically detects ME1 in WB, IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for ME1 antibody (ABIN7464215)

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ME1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Grade: KO Validated

Clone: GT15611

Product Details anti-ME1 Antibody

Cross-Reactivity: Human, Mouse

Purification: Affinity purified by Protein A.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ME1. The exact sequence is proprietary.

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ME1

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Alternative Name: malic enzyme 1

Background: Malic enzyme 1 , HUMNDME , MES,This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq]

Molecular Weight: 64 kDa

Gene ID: 4199

UniProt: P48163

Pathways: Regulation of Lipid Metabolism by PPARalpha