Vimentin antibody

Catalog No. ABIN7464236

This anti-Vimentin antibody is a Mouse Monoclonal antibody detecting Vimentin in WB, ELISA, IF, FACS, IHC (p), ICC, IP and IHC (fro). Suitable for Human.

Quick Overview for Vimentin antibody (ABIN7464236)

Target: Vimentin (VIM)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Grade: KO Validated

Clone: GT812

Product Details anti-Vimentin Antibody

Specificity: IP/MS validation was supported by references (PMID:30377427)

Cross-Reactivity: Cat, Human, Mouse, Rat

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:500-1:75000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , HeLa , U87-MG , SK-N-SH , IMR32 , SK-N-AS , Rat-2 , Neuro2A , C8D30 , NIH-3T3 , C2C12

Validation: Comparison, IP/MS, KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: vimentin

Background: Vimentin,Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Molecular Weight: 54 kDa

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis