Nibrin antibody

Catalog No. ABIN7464244

The Mouse Monoclonal anti-Nibrin antibody has been validated for WB, IF, ICC and IP. It is suitable to detect Nibrin in samples from Human.

Quick Overview for Nibrin antibody (ABIN7464244)

Target: Nibrin (NBN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nibrin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Grade: KO Validated

Clone: 1C3

Product Details anti-Nibrin Antibody

Cross-Reactivity: Human, Mouse

Purification: Protein G purified

Immunogen: The complete coding region of the human p95/NBS1 expressed in E. coli.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , MCF7 , HeLa , Raji

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Nibrin

Target: Nibrin (NBN)

Alternative Name: nibrin

Background: Nibrin , AT-V1 , AT-V2 , ATV , NBS , NBS1 , P95,Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008]

Molecular Weight: 85 kDa

Gene ID: 4683

UniProt: O60934

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response