ROCK2 antibody

Catalog No. ABIN7464345

This Mouse Monoclonal antibody specifically detects ROCK2 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for ROCK2 antibody (ABIN7464345)

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ROCK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Clone: GT11712

Product Details anti-ROCK2 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A549 , 293T , A431 , HeLa , HepG2

Validation: Comparison, KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ROCK2

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Alternative Name: Rho associated coiled-coil containing protein kinase 2

Background: Rho associated coiled-coil containing protein kinase 2 , ROCK-II,The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]

Molecular Weight: 161 kDa

Gene ID: 9475

UniProt: O75116

Pathways: Microtubule Dynamics, WNT Signaling, Tube Formation