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Vimentin antibody

This Mouse Monoclonal antibody specifically detects Vimentin in WB, ELISA, IF, IHC (p), ICC and IHC (fro). It exhibits reactivity toward Human.
Catalog No. ABIN7464381

Quick Overview for Vimentin antibody (ABIN7464381)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Reactivity

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Human

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This Vimentin antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Grade

KO Validated

Clone

GT7812
  • Cross-Reactivity

    Cat, Dog, Human, Mouse, Rat

    Purification

    Affinity purified by Protein G.

    Immunogen

    Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

    Isotype

    IgG2a
  • Application Notes

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    Comment

    Positive Control: C8D30 , NIH-3T3 , C2C12 , rat brain , 293T , NT2D1

    Validation: Comparison, KO/KD, Orthogonal, Overexpression

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.64 mg/mL

    Buffer

    PBS, No Preservative

    Preservative

    Without preservative

    Storage

    4 °C,-20 °C

    Storage Comment

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target

    Vimentin (VIM)

    Alternative Name

    vimentin

    Background

    Vimentin,Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

    Molecular Weight

    54 kDa

    Gene ID

    7431

    UniProt

    P08670

    Pathways

    Caspase Cascade in Apoptosis
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