BUB3 antibody

Catalog No. ABIN7464401

This anti-BUB3 antibody is a Rabbit Polyclonal antibody detecting BUB3 in WB, IHC (p) and IP. Suitable for Human.

Quick Overview for BUB3 antibody (ABIN7464401)

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-BUB3 Antibody

Specificity: IP/MS validation was supported by references (PMID:30377413)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human BUB3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: H1299 , 293T , A431 , HeLa , HepG2 , Neuro 2A , Raw264.7 , C2C12 , PC-12 , Rat2

Validation: IP/MS

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.32 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for BUB3

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Alternative Name: BUB3 mitotic checkpoint protein

Background: BUB3 mitotic checkpoint protein , BUB3L , hBUB3,This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Molecular Weight: 37 kDa

Gene ID: 9184

UniProt: O43684

Pathways: Maintenance of Protein Location