Villin 1 antibody (N-Term)

Catalog No. ABIN7464413

This anti-Villin 1 antibody is a Rabbit Polyclonal antibody detecting Villin 1 in WB, IF, ELISA, IHC (p) and ICC. Suitable for Human.

Quick Overview for Villin 1 antibody (N-Term) (ABIN7464413)

Target: Villin 1 (VIL1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Villin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-Villin 1 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Villin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: H1299 , mouse kidney , HepG2

Validation: Comparison

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.36 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7), 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Villin 1

Target: Villin 1 (VIL1)

Alternative Name: villin 1

Background: Villin 1 , D2S1471 , VIL,This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed, they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq]

Molecular Weight: 93 kDa

Gene ID: 7429

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization