NCOR2 antibody (AA 994-1005)

Catalog No. ABIN7464419

This anti-NCOR2 antibody is a Mouse Monoclonal antibody detecting NCOR2 in WB, IP, IHC (fro) and ChIP. Suitable for Human.

Quick Overview for NCOR2 antibody (AA 994-1005) (ABIN7464419)

Target: NCOR2 (Nuclear Receptor Co-Repressor 2 (NCOR2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NCOR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Chromatin Immunoprecipitation (ChIP)

Grade: KO Validated

Clone: 1212

Product Details anti-NCOR2 Antibody

Binding Specificity: AA 994-1005

Cross-Reactivity: Human, Monkey

Purification: Protein G purified

Immunogen: A synthetic peptide from the human SMRT protein corresponding to amino acids 994-1005 (ASASDPHREKTQ) coupled to KLH.

Isotype: IgG1

Application Details

Application Notes: For ChIP: Use 3 μL for 10^6 cells. For IHC-Fr: Use at a dilution of 1:50. Immunohistochemical staining of SMRT in HeLa cells with this antibody results in nuclear staining. For WB: Use at a dilution of 1:250. Detects a band of approximately 220-250 kDa (predicted molecular weight: 274 kDa). Optimal dilutions/concentration should be determined by the researcher.

Comment:

Positive Control: HBL100

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NCOR2

Target: NCOR2 (Nuclear Receptor Co-Repressor 2 (NCOR2))

Alternative Name: nuclear receptor corepressor 2

Background: Nuclear receptor corepressor 2 , CTG26 , N-CoR2 , SMAP270 , SMRT , SMRTE , SMRTE-tau , TNRC14 , TRAC , TRAC-1 , TRAC1,This gene encodes a nuclear receptor co-repressor that mediates transcriptional silencing of certain target genes. The encoded protein is a member of a family of thyroid hormone- and retinoic acid receptor-associated co-repressors. This protein acts as part of a multisubunit complex which includes histone deacetylases to modify chromatin structure that prevents basal transcriptional activity of target genes. Aberrant expression of this gene is associated with certain cancers. Alternate splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Apr 2011]

Molecular Weight: 274 kDa

Gene ID: 9612

UniProt: Q9Y618

Pathways: Notch Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha