Geminin antibody

Catalog No. ABIN7464441

This Rabbit Polyclonal antibody specifically detects Geminin in WB, IF, IP, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Geminin antibody (ABIN7464441)

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Geminin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Geminin Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Full length human Geminin Recombinant protein.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HCT116

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Geminin

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Alternative Name: geminin DNA replication inhibitor

Background: Geminin DNA replication inhibitor , Gem , MGORS6,Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.

Molecular Weight: 24 kDa

Gene ID: 51053

UniProt: O75496

Pathways: EGFR Signaling Pathway, DNA Replication