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Vimentin antibody (pSer56)

This anti-Vimentin antibody is a Rabbit Polyclonal antibody detecting Vimentin in WB. Suitable for Human.
Catalog No. ABIN7464482

Quick Overview for Vimentin antibody (pSer56) (ABIN7464482)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Reactivity

  • 376
  • 130
  • 128
  • 63
  • 61
  • 49
  • 43
  • 38
  • 30
  • 22
  • 21
  • 13
  • 13
  • 8
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  • 6
  • 6
  • 3
  • 3
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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Host

  • 198
  • 164
  • 11
  • 6
  • 6
  • 5
  • 2
  • 2
Rabbit

Clonality

  • 203
  • 190
Polyclonal

Conjugate

  • 240
  • 19
  • 13
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  • 9
  • 7
  • 6
  • 5
  • 5
  • 5
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  • 5
  • 3
  • 3
  • 3
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  • 3
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  • 3
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  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
This Vimentin antibody is un-conjugated

Application

  • 327
  • 170
  • 126
  • 106
  • 94
  • 63
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  • 40
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  • 34
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  • 23
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  • 1
  • 1
  • 1
  • 1
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Western Blotting (WB)

Grade

KO Validated
  • Binding Specificity

    • 34
    • 32
    • 17
    • 16
    • 12
    • 10
    • 8
    • 7
    • 5
    • 3
    • 3
    • 2
    • 2
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer56

    Cross-Reactivity

    Human

    Purification

    Purified by antigen-affinity chromatography.

    Immunogen

    Carrier-protein conjugated synthetic peptide surrounding phospho Ser56 of human Vimentin. The exact sequence is proprietary.

    Isotype

    IgG
  • Application Notes

    WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    Comment

    Positive Control: HeLa (100 ng/ml Nocodazole 24 hr) , 293T , NT2D1

    Validation: KO/KD, Orthogonal

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.3 mg/mL

    Buffer

    1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target

    Vimentin (VIM)

    Alternative Name

    vimentin

    Background

    Vimentin,Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

    Molecular Weight

    54 kDa

    Gene ID

    7431

    UniProt

    P08670

    Pathways

    Caspase Cascade in Apoptosis
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