ERN1 antibody (pSer724)

Catalog No. ABIN7464485

This anti-ERN1 antibody is a Rabbit Polyclonal antibody detecting ERN1 in WB. Suitable for Human.

Quick Overview for ERN1 antibody (pSer724) (ABIN7464485)

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ERN1 Antibody

Binding Specificity: pSer724

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Ser724 of human IRE1 alpha. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa (100 nM Calyculin A , 30 min)

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.18 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ERN1

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Alternative Name: endoplasmic reticulum to nucleus signaling 1

Background: Endoplasmic reticulum to nucleus signaling 1 , IRE1 , IRE1P , IRE1a , hIRE1p,The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq]

Molecular Weight: 110 kDa

Gene ID: 2081

UniProt: O75460

Pathways: ER-Nucleus Signaling, Unfolded Protein Response