Cyclin B1 antibody

Catalog No. ABIN7464490

The Mouse Monoclonal anti-Cyclin B1 antibody is suitable to detect Cyclin B1 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for Cyclin B1 antibody (ABIN7464490)

Target: Cyclin B1 (CCNB1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: GT238

Product Details anti-Cyclin B1 Antibody

Cross-Reactivity: Human

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Cyclin B1. The exact sequence is proprietary.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa (2mM thymidine treatment and recovery for 3.5hr) , HeLa (2mM thymidine treatment and recovery for 12hr) , U2OS (100ng/ml Nocodazole treatment for 24hr)

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: cyclin B1

Background: Cyclin B1 , CCNB,The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]

Molecular Weight: 48 kDa

Gene ID: 891

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase