SOX2 antibody

Catalog No. ABIN7464615

This Mouse Monoclonal antibody specifically detects SOX2 in WB, IF, FACS, ICC, IHC (p), IP and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for SOX2 antibody (ABIN7464615)

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Grade: KO Validated

Clone: GT1876

Product Details anti-SOX2 Antibody

Cross-Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:10000. IHC-Fr: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: human ESC , zebrafish eye , NT2D1 , PC-3 , U87-MG , SK-N-SH

Validation: Comparison, KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Alternative Name: SRY-box 2

Background: SRY-box 2 , ANOP3 , MCOPS3,This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]

Molecular Weight: 34 kDa

Gene ID: 6657

UniProt: P48431

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis