X-Ray Repair Cross Complementing 5 (C-Term) antibody

Catalog No. ABIN7464656

Product Details

Target: X-Ray Repair Cross Complementing 5

Binding Specificity: C-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Cross-Reactivity: Human, Monkey

Purification: Protein G purified

Grade: KO Validated

Immunogen: Recombinant antigen containing the carboxy terminal portion of human Ku80 protein

Clone: 149-8

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details

Target: X-Ray Repair Cross Complementing 5

Synonyms: KARP-1 antibody, KARP1 antibody, KU80 antibody, KUB2 antibody, Ku86 antibody, NFIV antibody, AI314015 antibody, Ku80 antibody, X-ray repair cross complementing 5 antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 antibody, XRCC5 antibody, Xrcc5 antibody

Background: X-ray repair cross complementing 5 , KARP-1 , KARP1 , KU80 , KUB2 , Ku86 , NFIV,The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]

Molecular Weight: 83 kDa

Gene ID: 7520

UniProt: P13010