BCLAF1 antibody

Catalog No. ABIN7464694

The Rabbit Polyclonal anti-BCLAF1 antibody has been validated for WB, IHC (p), IF and ICC. It is suitable to detect BCLAF1 in samples from Human.

Quick Overview for BCLAF1 antibody (ABIN7464694)

Target: BCLAF1 (BCL2-Associated Transcription Factor 1 (BCLAF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCLAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-BCLAF1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human BTF . The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: K562 nuclear extract

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.08 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for BCLAF1

Target: BCLAF1 (BCL2-Associated Transcription Factor 1 (BCLAF1))

Alternative Name: BCL2 associated transcription factor 1

Background: BCL2 associated transcription factor 1 , BTF , bK211L9.1,This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Molecular Weight: 106 kDa

Gene ID: 9774

UniProt: Q9NYF8

Pathways: Positive Regulation of Response to DNA Damage Stimulus