LC3B antibody (N-Term)

Catalog No. ABIN7464696

This Mouse Monoclonal antibody specifically detects LC3B in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for LC3B antibody (N-Term) (ABIN7464696)

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LC3B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: GT3612

Product Details anti-LC3B Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human

Purification: Affinity purified by Protein A.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary.

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: LC3B-transfected 293T , HepG2 (3 μM Thapsigargin treated for 12hr) , HeLa (50 μM chloroquine treated for 24hr)

Validation: Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: microtubule associated protein 1 light chain 3 beta

Background: Microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B-a,The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq]

Molecular Weight: 15 kDa

Gene ID: 81631

UniProt: Q9GZQ8

Pathways: Autophagy