FEN1 antibody (AA 1-380)

Catalog No. ABIN7464723

This Mouse Monoclonal antibody specifically detects FEN1 in WB, IF, IP, ICC and ChIP. It exhibits reactivity toward Human.

Quick Overview for FEN1 antibody (AA 1-380) (ABIN7464723)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FEN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Grade: KO Validated

Clone: 4-00E-07

Product Details anti-FEN1 Antibody

Binding Specificity: AA 1-380

Cross-Reactivity: Human, Mouse

Purification: Protein G purified

Immunogen: Recombinant human FEN-1 protein encoding amino acids 1-380 purified E. coli.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MCF7

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.73 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Alternative Name: flap structure-specific endonuclease 1

Background: Flap structure-specific endonuclease 1 , FEN-1 , MF1 , RAD2,The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]

Molecular Weight: 43 kDa

Gene ID: 2237

UniProt: P39748

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA