EEA1 antibody (C-Term)

Catalog No. ABIN7464759

This Rabbit Polyclonal antibody specifically detects EEA1 in WB, IF, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for EEA1 antibody (C-Term) (ABIN7464759)

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EEA1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Hamster, Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EEA1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , PC-12 , Rat2 , HepG2 , A375

Validation: Comparison, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EEA1

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Alternative Name: early endosome antigen 1

Background: Early endosome antigen 1 , MST105 , MSTP105 , ZFYVE2,Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

Molecular Weight: 162 kDa

Gene ID: 8411

UniProt: Q15075

Pathways: Toll-Like Receptors Cascades