PARN antibody

Catalog No. ABIN7464840

This Rabbit Polyclonal antibody specifically detects PARN in WB and IP. It exhibits reactivity toward Human.

Quick Overview for PARN antibody (ABIN7464840)

Target: PARN (Poly A Specific Ribonuclease (PARN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARN antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Grade: KO Validated

Product Details anti-PARN Antibody

Specificity: Knockdown/Knockout validation was supported by references (PMID:26990993)

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PARN. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , BCL-1

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PARN

Target: PARN (Poly A Specific Ribonuclease (PARN))

Alternative Name: poly(A)-specific ribonuclease

Background: Poly(A)-specific ribonuclease , DAN , DKCB6 , PFBMFT4,The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Molecular Weight: 73 kDa

Gene ID: 5073

UniProt: O95453