GAPDH antibody

Catalog No. ABIN7464846

This Mouse Monoclonal antibody specifically detects GAPDH in WB, IF, ICC, IHC (p) and EMSA. It exhibits reactivity toward Human.

Quick Overview for GAPDH antibody (ABIN7464846)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Electrophoretic Mobility-Shift Assay (EMSA)

Clone: GT239

Product Details anti-GAPDH Antibody

Cross-Reactivity: Candida albicans, Dog, Drosophila melanogaster, E. coli, Human, Monkey, Mouse, Pig, Rat, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , mouse brain , PC-12 , rat brain , coli M15 , 293T , A431 , HeLa , HepG2

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: glyceraldehyde-3-phosphate dehydrogenase

Background: Glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP,The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]

Molecular Weight: 36 kDa

Gene ID: 2597

UniProt: P04406