Dihydrofolate Reductase antibody

Catalog No. ABIN7464876

This Rabbit Polyclonal antibody specifically detects Dihydrofolate Reductase in WB, IF, IHC (p), IP and ICC. It exhibits reactivity toward Human.

Quick Overview for Dihydrofolate Reductase antibody (ABIN7464876)

Target: Dihydrofolate Reductase (DHFR)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dihydrofolate Reductase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-Dihydrofolate Reductase Antibody

Cross-Reactivity: Human, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DHFR. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: PC-12

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.54 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: dihydrofolate reductase

Background: Dihydrofolate reductase , DHFRP1 , DYR,Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. [provided by RefSeq]

Molecular Weight: 21 kDa

Gene ID: 1719

UniProt: P00374

Pathways: Mitotic G1-G1/S Phases