GRP78 antibody (C-Term)

Catalog No. ABIN7464913

The Rabbit Polyclonal anti-GRP78 antibody is suitable to detect GRP78 in samples from Mouse. It has been validated for WB, ICC, IF and IHC (p).

Quick Overview for GRP78 antibody (C-Term) (ABIN7464913)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRP78 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-GRP78 Antibody

Binding Specificity: C-Term

Specificity: Knockdown/Knockout validation was supported by customer review data.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Grp78. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Rat heart , Rat Lung , Rat Liver , Rat kidney , NIH-3T-3 , JC , BCL-1 , C2C12 , Raw264.7 , 293T , A431 , HeLa , HepG2

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.139 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: heat shock protein 5

Background: Heat shock protein 5 , AL022860 , AU019543 , Bip , D2Wsu141e , D2Wsu17e , Grp78 , Hsce70 , SEZ-7 , Sez7 , baffled , mBiP,Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.

Molecular Weight: 72 kDa

Gene ID: 14828

UniProt: P20029

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling