MBNL1 antibody

Catalog No. ABIN7464978

This Rabbit Polyclonal antibody specifically detects MBNL1 in WB, IF, ICC and IP. It exhibits reactivity toward Human.

Quick Overview for MBNL1 antibody (ABIN7464978)

Target: MBNL1 (Muscleblind-like Protein 1 (MBNL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBNL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Grade: KO Validated

Product Details anti-MBNL1 Antibody

Specificity: Knockdown/Knockout validation was supported by references (PMID:26990993)

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Muscleblind-like 1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U87-MG

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MBNL1

Target: MBNL1 (Muscleblind-like Protein 1 (MBNL1))

Alternative Name: muscleblind like splicing regulator 1

Background: Muscleblind like splicing regulator 1 , EXP , MBNL,Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.

Molecular Weight: 42 kDa

Gene ID: 4154

UniProt: Q9NR56

Pathways: Ribonucleoprotein Complex Subunit Organization