IARS antibody

Catalog No. ABIN7465193

Product Details anti-IARS Antibody

Target: IARS (Isoleucyl-tRNA Synthetase (IARS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IARS antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human IARS. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.45 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for IARS

Target: IARS (Isoleucyl-tRNA Synthetase (IARS))

Alternative Name: isoleucyl-tRNA synthetase (IARS Products)

Synonyms: fi46h05 antibody, zgc:63790 antibody, wu:fi46h05 antibody, K19E20.18 antibody, K19E20_18 antibody, ovule abortion 2 antibody, ECK0027 antibody, ilvS antibody, JW0024 antibody, An08g06770 antibody, AO090012000505 antibody, 2510016L12Rik antibody, AI327140 antibody, AU044614 antibody, E430001P04Rik antibody, ILRS antibody, Iarsl antibody, IARS1 antibody, ILERS antibody, IRS antibody, PRO0785 antibody, isoleucyl-tRNA synthetase antibody, tRNA synthetase class I (I, L, M and V) family protein antibody, isoleucine--tRNA ligase antibody, isoleucyl-tRNA synthetase, cytoplasmic antibody, isoleucine-tRNA synthetase antibody, iars antibody, IARS antibody, OVA2 antibody, ileS antibody, MBAR_RS19160 antibody, Rmag_0340 antibody, ANI_1_964074 antibody, CHLREDRAFT_106327 antibody, EDI_049880 antibody, OE_RS08520 antibody, AOR_1_1912194 antibody, Iars antibody

Background: Isoleucyl-tRNA synthetase , GRIDHH , IARS1 , ILERS , ILRS , IRS , PRO0785,Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Two alternatively spliced variants have been isolated that represent alternate 5' UTRs. [provided by RefSeq]

Molecular Weight: 144 kDa

Gene ID: 3376

UniProt: P41252