Cullin 4B antibody

Catalog No. ABIN7465207

The Rabbit Polyclonal anti-Cullin 4B antibody has been validated for WB, IF, IHC (p) and ICC. It is suitable to detect Cullin 4B in samples from Human.

Quick Overview for Cullin 4B antibody (ABIN7465207)

Target: Cullin 4B (CUL4B)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 4B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-Cullin 4B Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Cullin 4b. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , A375 , Neuro2A , C8D30 , NIH-3T3 , Raw 264.7 , C2Cl2 , PC-12 , Rat2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Cullin 4B

Target: Cullin 4B (CUL4B)

Alternative Name: cullin 4B

Background: Cullin 4B , CUL-4B , MRXHF2 , MRXS15 , MRXSC , SFM2,This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Molecular Weight: 104 kDa

Gene ID: 8450

UniProt: Q13620