EIF3H antibody

Catalog No. ABIN7465415

This anti-EIF3H antibody is a Mouse Monoclonal antibody detecting EIF3H in WB. Suitable for Human.

Quick Overview for EIF3H antibody (ABIN7465415)

Target: EIF3H (Eukaryotic Translation Initiation Factor 3 Subunit H (EIF3H))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF3H antibody is un-conjugated

Application: Western Blotting (WB)

Clone: GT2712

Product Details anti-EIF3H Antibody

Cross-Reactivity: Human

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human eIF3H / EIF3S3. The exact sequence is proprietary.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EIF3H

Target: EIF3H (Eukaryotic Translation Initiation Factor 3 Subunit H (EIF3H))

Alternative Name: eukaryotic translation initiation factor 3 subunit H

Background: Eukaryotic translation initiation factor 3 subunit H , EIF3S3 , eIF3-gamma , eIF3-p40,Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

Molecular Weight: 40 kDa

Gene ID: 8667

UniProt: O15372

Pathways: Ribonucleoprotein Complex Subunit Organization