EIF3H antibody

Catalog No. ABIN7465416

This Mouse Monoclonal antibody specifically detects EIF3H in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for EIF3H antibody (ABIN7465416)

Target: EIF3H (Eukaryotic Translation Initiation Factor 3 Subunit H (EIF3H))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF3H antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Clone: GT35512

Product Details anti-EIF3H Antibody

Cross-Reactivity: Human, Mouse

Purification: Affinity purified by Protein A.

Immunogen: Recombinant protein encompassing a sequence within the center region of human eIF3H / EIF3S3. The exact sequence is proprietary.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EIF3H

Target: EIF3H (Eukaryotic Translation Initiation Factor 3 Subunit H (EIF3H))

Alternative Name: eukaryotic translation initiation factor 3 subunit H

Background: Eukaryotic translation initiation factor 3 subunit H , EIF3S3 , eIF3-gamma , eIF3-p40,Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

Molecular Weight: 40 kDa

Gene ID: 8667

UniProt: O15372

Pathways: Ribonucleoprotein Complex Subunit Organization