MED4 antibody (N-Term)

Catalog No. ABIN7465497

This Rabbit Polyclonal antibody specifically detects MED4 in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for MED4 antibody (N-Term) (ABIN7465497)

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MED4 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MED4. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A549

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.9 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MED4

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Alternative Name: mediator complex subunit 4

Background: Mediator complex subunit 4 , ARC36 , DRIP36 , HSPC126 , TRAP36 , VDRIP,The Mediator is a multiprotein coactivator that is required by DNA-binding transcription factors for activation of polymerase II (see MIM 180660)-transcribed genes. MED4 appears to be a core Mediator subunit and is found in nearly all Mediator preparations (summary by Sato et al., 2004 [PubMed 15175163]).[supplied by OMIM]

Molecular Weight: 30 kDa

Gene ID: 29079

UniProt: Q9NPJ6

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha