NCK2 antibody

Catalog No. ABIN7465503

This Rabbit Polyclonal antibody specifically detects NCK2 in WB. It exhibits reactivity toward Human.

Quick Overview for NCK2 antibody (ABIN7465503)

Target: NCK2 (NCK Adaptor Protein 2 (NCK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCK2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NCK2 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Nck beta. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:20000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A549

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NCK2

Target: NCK2 (NCK Adaptor Protein 2 (NCK2))

Alternative Name: NCK adaptor protein 2

Background: NCK adaptor protein 2 , GRB4 , NCKbeta,This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Molecular Weight: 43 kDa

Gene ID: 8440

UniProt: O43639

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization