PICALM antibody

Catalog No. ABIN7465546

The Rabbit Polyclonal anti-PICALM antibody has been validated for WB and IHC (p). It is suitable to detect PICALM in samples from Human.

Quick Overview for PICALM antibody (ABIN7465546)

Target: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PICALM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PICALM Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CALM. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A549

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PICALM

Target: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Alternative Name: phosphatidylinositol binding clathrin assembly protein

Background: Phosphatidylinositol binding clathrin assembly protein , CALM , CLTH , LAP,Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.

Molecular Weight: 71 kDa

Gene ID: 8301

UniProt: Q13492

Pathways: Synaptic Membrane