DHCR7 antibody (C-Term)

Catalog No. ABIN7466581

The Rabbit Polyclonal anti-DHCR7 antibody has been validated for WB and IHC (p). It is suitable to detect DHCR7 in samples from Human.

Quick Overview for DHCR7 antibody (C-Term) (ABIN7466581)

Target: DHCR7 (7-Dehydrocholesterol Reductase (DHCR7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DHCR7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-DHCR7 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human DHCR7. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.86 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for DHCR7

Target: DHCR7 (7-Dehydrocholesterol Reductase (DHCR7))

Alternative Name: 7-dehydrocholesterol reductase

Background: 7-dehydrocholesterol reductase , SLOS,The DHCR7 gene encodes delta-7-sterol reductase (EC 1.3.1.21), the penultimate enzyme of mammalian sterol biosynthesis that converts 7-dehydrocholesterol (7-DHC) to cholesterol. This enzyme removes the C(7-8) double bond introduced by the sterol delta8-delta7 isomerases. In addition, its role in drug-induced malformations is known: inhibitors of the last step of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development (Moebius et al., 1998 [PubMed 9465114]).[supplied by OMIM]

Molecular Weight: 54 kDa

Gene ID: 1717

UniProt: Q9UBM7